Effects of Heterozygous Deletion of Autism-related Gene Cullin-3 in Mice

Qiangqiang Xia 08fa1c41e2 Update 'datacite.yml' 1 rok temu
Figure 1.pzfx 31c98b5396 Figure 1 WB 1 rok temu
Figure 2.pzfx 73d14b1c62 Figure 2 Motor Sensory Anxiety 1 rok temu
Figure 3 & Figure S2.pzfx 11e749d55c Figure 3 Repetitive Social 1 rok temu
Figure 4.pzfx 84e77b0583 Figure 4 Object Recognition 1 rok temu
Figure 5.pzfx a118640a99 Figure 5 Golgi Staining-Spine Density and Sholl Analysis 1 rok temu
Figure 6.pzfx 43f0f100f9 Figure 6 Global Cul3 Field Recording 1 rok temu
Figure 7.pzfx 8e0da13841 Figure 7 Cul3 global mEPSC &NMDA-AMPA ratio 1 rok temu
Figure 8.pzfx 8ba51da972 Figure 8 Proteomic Analysis 1 rok temu
Figure S1.pzfx ca29e28483 Figure S1. Original uncropped and unadjusted western blot results from Figure 1B. 1 rok temu
Figure S3.pzfx 66404c5438 Figure S3. Original uncropped and unadjusted western blot results from Figure 8G 1 rok temu
LICENSE 51ace4e976 Initial commit 1 rok temu
Proteomics Raw data-Powell Lab.zip 5c68e79ebf Upload files to '' 1 rok temu
README.md 8480cb3e3b Update 'README.md' 1 rok temu
Supplementary material.docx afb00e2262 Supplementary material 1 rok temu
datacite.yml 08fa1c41e2 Update 'datacite.yml' 1 rok temu

README.md

PONE-D-22-26810

Title: Effects of Heterozygous Deletion of Autism-related Gene Cullin-3 in Mice

Description: This is a original dataset repository for manuscript # PONE-D-22-26810 published at PLOS ONE, including the Western blot (Figure 1 and Figure S1/S3), behavioral tests (Figure 2-4 and Figure S2, Supplementary material), Golgi staining (Figure 5), field recording (Figure 6), whole-cell patch clamp recording (Figure 7), proteomic analysis (figure 8 and proteomic raw data file .zip).

The figure files format are .pzfx, which can be open by Prism GraphPad: https://www.graphpad.com/features; these figure files including the raw data of these Western blots, behavioral tests, Golgi stainings, electrophysiological recordings etc. at Data tables; statistical analysis at Results; graphs plotted from these raw data at Graphs; figures made from these grpahs at Layouts.

Proteomic raw data file .zip can be uncompressed by WinRAR: https://www.win-rar.com/start.html?&L=0, which consists of upregulated and downregulated expressed proteins after Cul3 heterozygous deletion in mouse brains, a powerpoint .ppt that can be opened by Office powerpoint analyzed, explpained and summarized the Proteomic raw data.

Supplementary material is a .word that can be opened by Office Word, which including the figure legends of Figure S1-S3.

please contact qqxia@uab.edu if you have any questions on these data.

datacite.yml
Title Effects of Heterozygous Deletion of Autism-related Gene Cullin-3 in Mice
Authors Xia,Qiang-qiang;University of Alabama at Birmingham;ORCID:0000-0002-8490-6437
Powell,Craig M.;University of Alabama at Birmingham;ORCID:0000-0001-5451-2165
Description Cul3 heterozygous deletion impairs spatial object recognition memory, alters cytoskeletal organization proteins, but does not cause major hippocampal neuronal morphology, functional, or behavioral abnormalities in adult global Cul3 heterozygous mice
License Creative Commons CC0 1.0 Public Domain Dedication (https://creativecommons.org/publicdomain/zero/1.0/)
References title: Effects of Heterozygous Deletion of Autism-related Gene Cullin-3 in Mice; authors: Qiang-qiang Xia, ... , Craig M. Powell; journal: PLOS ONE [doi:tba] (IsReferencedBy)
Funding National Institute of Mental Health (NIMH); R01 MH113948
Keywords autism
behavior
cullin-3
Resource Type Dataset