S1S2_all-optical_data

S1S2_all-optical_data

Calcium imaging data of primary & secondary somatosensory cortex (S1, S2) in mice, while simultaneously optogenetically stimulating S1 neurons.

This data corresponds to the following publication and preprint. Please cite it if using these data:

Rowland, Van der Plas, Loidolt et al., Propagation of activity through the cortical hierarchy and perception are determined by neural variability, Nature Neuroscience (2023).

Or find our preprint at: https://www.biorxiv.org/content/10.1101/2021.12.28.474343v2

Code to easily load the data:

To load the all optical imaging/optogenetics data, it is easiest to use the Python data Class that we developed. Please find an example notebook at:

https://github.com/Packer-Lab/popping-off/blob/master/notebooks/Example%20notebook%20to%20load%20data.ipynb

To load the pupil data, it is easiest to use the functions and examples in the following notebook:

https://github.com/Packer-Lab/popping-off/blob/master/notebooks/Paper%20Figures/Pupil%20analysis.ipynb

Description of data:

sessions_lite_flu_2022-08-11.pkl

• description: these are the all-optical recordings of N=11 recording sessions. For each session, neural activity was recorded in S1 and S2, while neurons in S1 were optogenetically stimulated. Behaviour (in the form of licking a water spout) was simultaneously recorded. See above for an example notebook to load these data.
• file format: .pkl (compressed Python data)

pupil_size_data/*

• description: folder with the pupil size arrays of N=3 recording sessions (separate files). Pupil size was recorded on video and pre-processed such that these files report an array of pupil size values. See above for an example notebook to load these data.
• file format: .npy (numpy array)

source_data_images/images_edf10/*

• description: two source images used for Fig. 1b,c.
• file format: .jpg

source_data_images/images_edf1/*

• description: four source images used for Extended Data Fig. 1b,c.
• file format: .jpg

source_data_images/images_edf10/*

• description: one image showing an example video frame of small & large pupil size. Used for Extended Data Fig. 10a.
• file format: .jpg