This repository contains the electrophysiological data and custom code related to the article “A longitudinal bilateral primary motor cortex electrophysiological and behavior dataset for hemi-parkinsonian rat in response to deep brain stimulation”. Here we provided the electrophysiological dataset collected from layer V of the primary motor cortex (M1) and the corresponding behavior dataset from normal and hemi-parkinson rats over 5 consecutive weeks. The electrophysiological dataset was constituted by the raw wideband signal, neuronal spikes, and local field potential (LFP) signal. Following the content of this article, the dataset was built according to the rat ID.
Freely moving recordings:
The data acquisition process was performed in a quiet test room, which was under a 12 h/12 h light-dark cycle. Rats were placed in a tailored Faraday cage with free access to food and water. The OmniPlex Neural Recording Data Acquisition System (Plexon Inc., Dallas, Texas, USA) was used to record the wideband electrophysiological data. Then, this system could filter the neural signals of spike and LFP from the wideband signal, which were selected using band-pass filter set at 300-3000 Hz and 0.5-200 Hz, respectively, and further digitized at a sampling rate of 40 kHz and 1 kHz. The wideband signal, spike, and LFP data files were all saved for further offline analysis.
Electrode channels:
In this study, the stimulation and recording electrodes were all microwire array electrodes. The recording microwire array was arranged in 4×4 array (nickel-titanium, 25 μm diameter, 300 μm interelectrode spacing, 8 mm length, Suzhou Kedou Brain Computer Technology Co., Ltd), and two silver wires with 125 μm diameter were reserved at the electrode connector as ground wires. The stimulation microwire array was arranged in 2×2 array (tungsten, 50 μm diameter, 250 μm interelectrode spacing, 10 mm length, Beijing Plexon Co., Ltd). Nissl staining was performed to determine the electrode position, only electrophysiological data of rats with correctly electrode implantation was used in subsequent analysis.
Behavioral assessment：
The open-field test was done to evaluate the behavior variation of rats among the entire experimental cycle, we provided the amount and duration of movement in the Excel spreadsheet to quantify the symptoms variation of rats and effects of closed-loop DBS.
Custom code：
We provided Python code for obtaining clean LFP signal from the wideband signal, as well as their power spectral analysis to ease the reproducibility of these demonstrations.
For every question and/or further data request, please don't hesitate in contacting me (drwangxiaofeng@126.com)
