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+---
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+title: "HCS demo"
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+output: html_notebook
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+---
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+
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+
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+# main figure
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+
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+The main figure is the result figure, what was achieved. In this case we will probably use a infographics:
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+
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+mutant/treated mice -> video taped 24h ->
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+
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+## status before:
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+
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+excel 1h summary data -> excel analysis of summary data (2-5h):
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+
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+- p-hacking and harking -> false positive
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+- 20 to 24h of data, all pooled -> variability and false pos/neg
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+- no circadian clock reseting - false neg.
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+- hugly graphs
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+
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+
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+## status after
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+
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+behavior sequence data -> metadata creation (1-2h) -> run script
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+
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+- data reset around light off event
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+- and cut at shortest period present for all mice,
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+- statistically sound analysis without a priori (PCA, SVM)
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+- 3D visualisation
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+- html/pdf Report
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+
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+- extendable (sequence analysis)
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+- FAIR data
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+
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+## Summary
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+
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+- faster, more powerful
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+- clean and statistically sound analysis
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+- FAIR data
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+
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