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- # Required fields
- # The main researchers involved working on the resource,
- # or the authors of the publication in priority order.
- # May be a corporate/institutional or personal name.
- # Include digital identifier (e.g., ORCID) if possible
- authors:
- -
- firstname: "Julia"
- lastname: "Gamache"
- affiliation: "N. Bud Grossman Center for Memory Research and Care, Department of Neurology, University of Minnesota, Minneapolis, MN 55414"
- id: "0000-0002-3750-147X"
- -
- firstname: "Kellie"
- lastname: "Benzow"
- affiliation: "Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN 55414"
- -
- firstname: "Colleen"
- lastname: "Forster"
- affiliation: "Histology & Research Laboratory, Clinical and Translational Science Institute, University of Minnesota, Minneapolis, 55414"
- -
- firstname: "Lisa"
- lastname: "Kemper"
- affiliation: "N. Bud Grossman Center for Memory Research and Care, Department of Neurology, University of Minnesota, Minneapolis, MN 55414"
- -
- firstname: "Chris"
- lastname: "Hlynialuk"
- affiliation: "N. Bud Grossman Center for Memory Research and Care, Department of Neurology, University of Minnesota, Minneapolis, MN 55414"
- -
- firstname: "Eva"
- lastname: "Furrow"
- affiliation: "Veterinary Clinical Sciences (College of Veterinary Medicine), University of Minnesota, Minneapolis, MN 55414"
- -
- firstname: "Karen H."
- lastname: "Ashe"
- affiliation: "N. Bud Grossman Center for Memory Research and Care, Department of Neurology, University of Minnesota, Minneapolis, MN 55414"
- -
- firstname: "Michael D."
- lastname: "Koob"
- affiliation: "Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN 55414"
-
- # A name or title to describe the published resource.
- title: Factors other than hTau overexpression that contribute to tauopathy-like phenotype in rTg4510 mice
- # Any additional information. It is best practice to supply a description for the resource.
- description: |
- The tauopathy-like phenotype observed in the rTg4510 mouse line, in which human tauP301L expression
- specifically within the forebrain can be temporally controlled, has largely been attributed to high overexpression
- of mutant human tau in the forebrain region. Unexpectedly, we found that in a different mouse line with a
- targeted-insertion of the same transgene driven by the same tetracycline-TransActivator (tTA) allele, but with
- even higher overexpression of tauP301L than rTg4510, atrophy and tau histopathology are delayed, and a
- different behavioral profile is observed. This suggests that it is not overexpression of mutant human tau alone
- that contributes to the phenotype in rTg4510 mice. Furthermore we show that the tauopathy-like phenotype
- seen in rTg4510 requires a ~70-copy tau-transgene insertion in a 244kb deletion in Fgf14, a ~7-copy tTA-
- transgene insertion in a 508kb deletion that disrupts another five genes, in addition to high transgene
- overexpression. We propose that these additional effects need to be accounted for in any studies using
- rTg4510, and that Tg-INDEL mutations and their impacts on phenotype should be defined for all transgenic
- models used in biomedical research.
-
- # List of keywords the resource should be associated with.
- keywords:
- - Neuroscience
- - Transgene
- - rTg4510
- - Tau
- - Fgf14
- # Any rights information for this resource. Please provide both a license name and a link to the license.
- # Please add also a LICENSE file to the repository
- license:
- name: "Creative Commons CC0 1.0 Public Domain Dedication"
- url: "https://creativecommons.org/publicdomain/zero/1.0/"
- ## Optional Fields
- # Any funding reference for this resource. Separate funder name and grant number by comma
- funding:
- # Related publications. reftype might be: IsCitedBy, IsSupplementTo, IsReferencedBy, IsPartOf
- # for further valid types see https://schema.datacite.org/meta/kernel-4
- # Please provide digital identifier (e.g., DOI) if possible.
- references:
- # Type of the data in this repository (Dataset, Model, Software, Other see
- # https://schema.datacite.org/meta/kernel-4.1/doc/DataCite-MetadataKernel_v4.1.pdf
- # for examples
- dtype: Source Data
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