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+# Required fields
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+# The main researchers involved working on the resource,
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+# or the authors of the publication in priority order.
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+# May be a corporate/institutional or personal name.
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+# Include digital identifier (e.g., ORCID) if possible
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+authors:
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+ -
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+ firstname: "Julia"
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+ lastname: "Gamache"
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+ affiliation: "N. Bud Grossman Center for Memory Research and Care, Department of Neurology, University of Minnesota, Minneapolis, MN 55414"
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+ id: "0000-0002-3750-147X"
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+ -
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+ firstname: "Kellie"
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+ lastname: "Benzow"
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+ affiliation: "Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN 55414"
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+ -
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+ firstname: "Colleen"
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+ lastname: "Forster"
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+ affiliation: "Histology & Research Laboratory, Clinical and Translational Science Institute, University of Minnesota, Minneapolis, 55414"
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+ -
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+ firstname: "Lisa"
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+ lastname: "Kemper"
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+ affiliation: "N. Bud Grossman Center for Memory Research and Care, Department of Neurology, University of Minnesota, Minneapolis, MN 55414"
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+ -
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+ firstname: "Chris"
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+ lastname: "Hlynialuk"
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+ affiliation: "N. Bud Grossman Center for Memory Research and Care, Department of Neurology, University of Minnesota, Minneapolis, MN 55414"
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+ -
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+ firstname: "Eva"
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+ lastname: "Furrow"
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+ affiliation: "Veterinary Clinical Sciences (College of Veterinary Medicine), University of Minnesota, Minneapolis, MN 55414"
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+ -
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+ firstname: "Karen H."
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+ lastname: "Ashe"
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+ affiliation: "N. Bud Grossman Center for Memory Research and Care, Department of Neurology, University of Minnesota, Minneapolis, MN 55414"
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+ -
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+ firstname: "Michael D."
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+ lastname: "Koob"
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+ affiliation: "Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN 55414"
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+
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+
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+# A name or title to describe the published resource.
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+title: Factors other than hTau overexpression that contribute to tauopathy-like phenotype in rTg4510 mice
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+
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+# Any additional information. It is best practice to supply a description for the resource.
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+description: |
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+ The tauopathy-like phenotype observed in the rTg4510 mouse line, in which human tauP301L expression
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+ specifically within the forebrain can be temporally controlled, has largely been attributed to high overexpression
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+ of mutant human tau in the forebrain region. Unexpectedly, we found that in a different mouse line with a
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+ targeted-insertion of the same transgene driven by the same tetracycline-TransActivator (tTA) allele, but with
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+ even higher overexpression of tauP301L than rTg4510, atrophy and tau histopathology are delayed, and a
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+ different behavioral profile is observed. This suggests that it is not overexpression of mutant human tau alone
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+ that contributes to the phenotype in rTg4510 mice. Furthermore we show that the tauopathy-like phenotype
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+ seen in rTg4510 requires a ~70-copy tau-transgene insertion in a 244kb deletion in Fgf14, a ~7-copy tTA-
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+ transgene insertion in a 508kb deletion that disrupts another five genes, in addition to high transgene
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+ overexpression. We propose that these additional effects need to be accounted for in any studies using
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+ rTg4510, and that Tg-INDEL mutations and their impacts on phenotype should be defined for all transgenic
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+ models used in biomedical research.
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+
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+# List of keywords the resource should be associated with.
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+keywords:
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+ - Neuroscience
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+ - Transgene
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+ - rTg4510
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+ - Tau
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+ - Fgf14
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+
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+# Any rights information for this resource. Please provide both a license name and a link to the license.
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+# Please add also a LICENSE file to the repository
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+license:
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+ name: "Creative Commons CC0 1.0 Public Domain Dedication"
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+ url: "https://creativecommons.org/publicdomain/zero/1.0/"
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+
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+## Optional Fields
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+
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+# Any funding reference for this resource. Separate funder name and grant number by comma
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+funding:
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+
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+# Related publications. reftype might be: IsCitedBy, IsSupplementTo, IsReferencedBy, IsPartOf
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+# for further valid types see https://schema.datacite.org/meta/kernel-4
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+# Please provide digital identifier (e.g., DOI) if possible.
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+references:
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+
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+# Type of the data in this repository (Dataset, Model, Software, Other see
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+# https://schema.datacite.org/meta/kernel-4.1/doc/DataCite-MetadataKernel_v4.1.pdf
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+# for examples
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+dtype: Source Data
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