5HT_pain_ephys_source/microscopy.json

{
    "PixelSize": [
        0.2,
        0.3
    ],
    "FieldOfView": [
        75,
        80
    ],
    "AcquisitionParameters": "NA: 1.4",
    "BodyPart": "BRAIN",
    "BodyPartDetails": "rostro ventro medial medulla",
    "SamplePrimaryAntibody": "polyclonal rabbit anti-serotonin antibody, (Ref. PC228L, batch D21789-1; Calbiochem, La Jolla, CA, USA)",
    "SampleSecondaryAntibody": "Alexa fluor 594 goat anti-rabbit antibody, (Invitrogen, Cergy Pontoise, France) to label serotonin, and streptavidin conjugated to Alexa fluor 488, (Invitrogen) to label neurobiotin",
    "SampleStaining": "DAB",
    "SliceThickness": "80",
    "SampleFixation": "exvivo",
    "SampleStainingDescription": "4% paraformaldehyde, 0.1% glutaraldehyde, 0.05% picric acid"
}