# Nrxn1 Knock-out
Longitudinal study monitoring the progression of any electrophysiological phenotype related to sensory processing in the Nrxn1α knock-out model.
The study contains two data sets (raw):
 - Charaterization (folder: charact) contains: 4-hour recordings at 10 weeks and 22 weeks, VEP and AEP at 10, 14, 18 and 22 weeks of age.
- Arbaclofen (folder: arbaclofen) contains: 24-hour recordings at 8 weeks and 22 weeks, cross-over study VEP and AEP with 2 mg/kg arbaclofen at 8 weeks and 22 weeks.

#### File types
The recordings were made in TainiLive. The recording files are of the .edf+ type, meta data is provided as .yaml files and screenshots of the recording logs are available containing the exact transmitter frequency of each recording. The 24-hour recordings contain data from motion sensors recorded with Spike2 (CED) as .smrx format.

## Characterization

#### Electrode configuration
Electrodes implanted bilaterally over visual (AP -4, ML +- 2.5) (ch 3 & 14) and auditory cortices (AP -2.5, ML +- 3.5) (ch 4 & 12) and unilaterally over PFC (AP 2.58 ML -1.57) (ch13). Two EMGs in neck muscles (ch 8 & 11) and reference over the cerebellum.

#### Recording specifications
Due to software updates during the study the sampling frequency is not the same in all recordings
- Sampling frequency: 19525 Hz
- Low-pass filter: 9700 Hz
- High-pass filter: 0.35 Hz


A few files were recorded with the sampling frequency: 2789.3 Hz
- VEP 3: 55970, 55971, 55963, 55964



#### Genotypes
The genotype of the animal is presented below:

| Batch # | WT | Het | Hom |
| -------- | -------- | -------- | -------- |
| 1       | 51333 |51334 |51372 |
|         |51346 |51341 |51383 |
|         |51355 |51343 |51389 |
|         |51363 |51344 |51391e|
|         |51375 |51345 |51403 |
|         |51413 |51351 |51405 |
|         |      |51352 |      |
| 2       |55970 |55949 |55948 |
|         |55979 |55963 |55964 |
|         |55984 |55983 |55971 |
|         |      |55985 |55978 |
| 3       |57738 |57767 |57737 |
|         |57753 |57781 |57752 |
|         |57760 |      |57762 |
|         |57763 |      |57766 |
|         |57782 |      |      |
|Total n  |  14  |  13  |13(14)|

e - poor signal at last recording

## Arbaclofen study
Longitudinal study of how acute administration of arbaclofen affect the Nrxn1α knock-out model. Arbaclofen (2 mg/kg) i saline was administered in a cross-over design at 8 weeks and 22 weeks of age.

The data includes a dose adjustment pilot (folder: VEP/additional/dose-response) with doses 3 mg/kg and 4 mg/kg and VEP gating at 30 weeks (folder: VEP/additional/aging).

#### Electrode configurations

Electrode configuration was the same as above for most of the animals, but for animals: 74979,74980,74981,74983,75023
The electrode configuration was as follows:

Electrodes implanted bilaterally over visual (AP -4, ML +- 2.5) (ch 14 & 4)  and auditory cortices (AP -2.5, ML +- 3.5) (ch 13 & 5) and unilaterally over PFC (AP 2.58 ML -1.57) (ch 3). Two EMGs in neck muscles (ch 8 & 11) and ref over the cerebellum.

#### Randomization
First recording (visual and auditory) of first time point:
- Arb: 75031, 74960, 74959, 74965, 74985, 74930, 74931, 74988, 74924, 74968, 75024
- Vehicle: 74979, 74980, 74981, 74983, 75032, 75033, 74958, 74966, 74967, 74986, 74989, 75023
- second recordings, vice versa

First recording of second time point:
- Arb: 74979, 75032, 75033, 74960, 74958, 74959, 74966, 74967, 74930, 74989, 74968
- Vehicle: 74980, 74981, 74983, 75031, 74965, 74985, 74931, 74988, 74924, 75024, 75023
- second recordings, vice versa

#### Recording specifications
- Sampling rate: 1084.7 Hz
- Low-pass filter: 9700 Hz
- High-pass filter: 0.35 Hz

#### Genotypes
| WT | Het | Hom |
| --- | --- | --- |
| 74959 | 74924 | 74930 |
| 74967 | 74960 | 74931 |
| 74968 | 74965 | 74958 |
| 74986e| 74966 | 74981 |
| 74988 | 74979 | 74983 |
| 75024 | 74980 | 74985 |
|       | 74989 | 75023 |
|       | 75032 | 75031 |
|       |       | 75033 |
|n=6 | 8 | 9 |

e - only first time point